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A Fluorescence Life-sciences research-grade microscope, the PA53 BIO FS6 delivers stunning optical performance at an affordable price. With a 6 channel fluorescence turret including DAPI, FITCI, TRITCI, & multiband FISH Filter Cubes. Motic’s high-quality optics provide high color fidelity and resolution with stunning 25mm widefield capability. A Motic camera and software will allow you to capture and save images as well as make measurements. A bright and long-lasting LED light option supplies excellent lighting for your samples.
From Simple Measurements to Complicated Analyses -- Our Superior Optical Performance Fulfills Your Needs
Fluorescence Module
The coded fluorescence turret automatically remembers the fluorescence channel and each channels brightness. This avoids the issue of stray light and saves you from having to purchase expensive OD6 or OD8 filters to increase fluorescence performance.
Motic Fluorescence Imaging Software
Our Motic Fluorescence Imaging Software plug-in for Motic Image Plus 3.0 is included with all PA53 FS6 purchases. It was developed in-house and offers a professional fluorescence digital image processing solution for multi-channel imaging. This software supports up to four channel imaging which can then be merged to meet FISH and other fluorescence imaging analysis applications.
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| Filters | DAPI | FITC | TRITC | RGB Three channels integration |
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| Model | PA53 BIO FS6 | PA53 BIO FS6 EDF |
| Optical System | Colour Corrected Infinity Optical System (CCIS®) | Colour Corrected Infinity Optical System (CCIS®) |
| Observation tube | Trinocular head, Siedentopf type | Trinocular head, Siedentopf type |
| Inclination | 30° inclined | 30° inclined |
| Trinocular light split | 100:0/20:80/0:100 | 100:0/20:80/0:100 |
| Interpupillary distance | 50-75mm | 50-75mm |
| Diopter adjustment | On both eyepieces, +/- 4 diopter | On both eyepieces, +/- 4 diopter |
| Eyepieces | Widefield WF10X/23mm with diopter adjustment | Widefield WF10X/23mm with diopter adjustment |
| Intermediate body | Epi-Fluorescence illuminator LUMOS LED with 6 position coded fluorescence turret and DAPI, FITC and TRITC filter cubes mounted | Epi-Fluorescence illuminator LUMOS LED with 6 position coded fluorescence turret and DAPI, FITC and TRITC filter cubes mounted |
| Nosepiece | Reversed quintuple, coded | Reversed quintuple, coded |
| Objective classification | CCIS® HPlan S-APO (Fluor) | CCIS® HPlan S-APO (Fluor) |
| Objectives | 4X/0.13 (WD 17.3mm), 10X/0.3 (WD 11.7mm), 20X/0.5 (WD 2.2mm), 40X/0.75/S (WD 0.7mm), 100X/1.3/S-Oil (WD 0.1mm) | 4X/0.13 (WD 17.3mm), 10X/0.3 (WD 11.7mm), 20X/0.5 (WD 2.2mm), 40X/0.75/S (WD 0.7mm), 100X/1.3/S-Oil (WD 0.1mm) |
| Objective mounting thread | W 4/5"x1/36" (RMS standard) | W 4/5"x1/36" (RMS standard) |
| Stand type | Upright | Upright. Auto EDF focusing function, motorized Z-axis (stroke 30mm) with control box and control pad |
| Stage | Mechanical stage with built-in low position rackless coaxial stage control and sample holder | Mechanical stage with built-in low position rackless coaxial stage control and sample holder |
| Stage size | 220x170mm | 220x170mm |
| Travel range X&Y | 80x55mm | 80x55mm |
| Condenser | Focusable and centerable Abbe condenser N.A. 0.90/1.25 with slot for contrast sliders | Focusable and centerable Abbe condenser N.A. 0.90/1.25 with slot for contrast sliders |
| Diaphragm | Iris diaphragm | Iris diaphragm |
| Focus mechanism | Coaxial coarse and fine focusing system with tension adjustment | Coaxial coarse and fine focusing system with tension adjustment |
| Fine focus precision | 1µm | 1µm |
| Focusing stroke | 29.5mm - Coarse: 17.7mm/revolution - Fine: 0.1mm/revolution (1µm scale) | 29.5mm - Coarse: 17.7mm/revolution - Fine: 0.1mm/revolution (1µm scale) |
| Upper limit stop | Upper limit stop preset but adjustable | Upper limit stop preset but adjustable |
| Filter | ND6, ND25, LBD | ND6, ND25, LBD |
| Filter holder | Integrated filters | Integrated filters |
| Incident illumination | LUMOS High-power LED FL light source (Optional: Mercury light source) | LUMOS High-power LED FL light source (Optional: Mercury light source) |
| Transmitted illumination | Köhler Quartz halogen 12V/100W with intensity control | Köhler Quartz halogen 12V/100W with intensity control |
| Illumination features | Power saving mode ECO function, LED voltage indicator and Intelligent Light function | Power saving mode ECO function, LED voltage indicator and Intelligent Light function |
| Software | Motic Images Plus 3.0 software with plug-in FL channel merge | Motic Images Plus 3.0 software with plug-in FL channel merge |
| Transformer | Internal | Internal |
| Power supply | 110-240V (CE) | 110-240V (CE) |
| Accessories included | Dust cover, power cord, Allen key, immersion oil (5ml), 2 empty filter cubes, UV light blocking tube, UV light protective shield | Dust cover, power cord, Allen key, immersion oil (5ml), 2 empty filter cubes, UV light blocking tube, UV light protective shield |
| Dimensions LxWxH | 648x247x513 | 648x247x513 |
| Net weight | 24.2kg | 29.7kg |
| Contrast techniques | ||
| Standard contrast technique | Brightfield | Brightfield |
| Phase contrast | Optional slider/turret | Optional slider/turret |
| Polarization | Optional add-on | Optional add-on |
| Darkfield | Optional slider/turret | Optional slider/turret |
| Fluorescence | LED | LED (HBO optional) |
